Additional file 12: of Genome-wide identification, phylogeny, evolution, and expression patterns of MtN3/saliva/SWEET genes and functional analysis of BcNS in Brassica rapa

Figure S6. Positive detection of transgenic Chinese cabbage (B. campestris ssp. chinensis var. parachinensis). A, PCR amplification detection. Lane 1, marker; Lane 2, amplification results of the negative control, water; Lane 3, amplification results of the positive control, 35S-pCAM1BIA1301; Lanes 4–8, amplification results of the positive control, 35S-pCAM1IA1301; Lanes 9–19 amplification results of the 35 s-BcNS transformants. B and C, X-Gluc histochemical staining detection of calluses and leaves. D, Fluorogenic quantitative PCR detection; WT, wild type; CK, negative control; A-12, A-13, and A-20, antisense expression plant. (TIFF 1610 kb