Additional file 1: Figure S1. of Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Alignments between B. napus and B. rapa TTG1 sequences, primer coverage, and binary vector construction for manipulating TTG1 in B. napus. B. napus TTG1-1 (EF175930), TTG1-2 (EF175932) and B. rapa TTG1 (HM208590.1). Figure S2. PCR confirmation of O-TTG1 or K-TTG1 TDNA in putative phosphinothricin tolerant T0 transformants of B. napus cv Westar and AtGL3+ B. napus. Figure S3. Representative Southern analysis to detect copy number of K-TTG1 and O-TTG1 TDNA in AtGL3+ B. napus and Westar. Figure S4. Relative expression of BnTTG1 in seedling tissues of T0 plants transformed with K-TTG1 or O-TTG1 binary vectors in semi-glabrous cv. Westar and hairy AtGL3+ B. napus backgrounds using Q-PCR. Figure S5. Fourth rosette leaf trichome phenotypes of two-week-old Arabidopsis thaliana SALK lines 074628 (BRG2), 127112C (BRG2) and 036510 (SKS2). Figure S6. Stem trichomes on T0 hairy B. napus K-5-8 at eight weeks after germination. Figure S7. Heatmap illustrating the relative expression levels and numbers of unique up-regulated and down-regulated genes (p