TnSeq reveals differences among strains in requirements for genes in central carbon metabolism pathways.

<p>Schematic of central carbon metabolism in Mtb highlighting genes with statistically significant differences in transposon insertion count between clinical strains and the reference strain H37Rv. Metabolites are represented by dots; enzymes are represented by diamonds and gene abbreviations are shown next to the reactions they catalyze. Diamonds with blue fill indicates at least one clinical strain has significantly more transposon insertions across the corresponding gene(s) as compared to H37Rv; red fill indicates at least one clinical strain has significantly fewer transposon insertions across the gene(s) as compared to H37Rv. Heatmaps show log₂ fold-change in transposon insertion count for this set of genes across all strains as indicated by the color scale; asterisk indicates statistical significance (Benjamini-Hochberg adjusted P < 0.05, fold-change ≥ 2 or ≤-2). Metabolite abbreviations: ACCOA, acetyl-CoA; ASP, aspartate; CIT, citrate; DHP, dihydroxyacetone phosphate; F6P, fructose-6-phosphate; FBP, fructose 1,6-bisphosphate; FUM, fumarate; G3P, glycerol 3-phosphate; G6P, glucose-6-phosphate; GA3P, glyceraldehyde 3-phosphate; GLUC, glucose; GLX, glyoxylate; GLYC, glycerol; ICIT, isocitrate/citrate; MAL, malate; OAA, oxaloacetate; OXG, 2-oxoglutarate; PEP, phosphoenolpyruvate; PYR, pyruvate; SUC, succinate; SUCCOA, succinyl-CoA. The metabolic pathway schematic was generated with Omix [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006939#ppat.1006939.ref063" target="_blank">63</a>].