The candidate genes are located mainly in the Embden–Meyerhof–Parnas (EMP) pathway and the TCA cycle, and are associated with amino acid metabolism, and fructose and mannose metabolism.

<p>Numerals refer to genes to be disrupted in this work: 1, hexokinase I; 2, hexokinase II; 3, sorbitol reductase; 4, sorbitol de- hydrogenase; 5, phosphoglycerate dehydrogenase; 6, phosphoglycerate dehydrogenase; 7, 3-phosphoserine aminotransferase; 8, 3-phospho- serine phosphatase; 9, L-alanine transaminase; 10, mitochondrial citrate transport; 11, isocitrate dehydrogenase. Abbreviations represent: Glu, glucose; Glu6P, glucose-6p; Fru6P, fructose-6p; Fru, fructose; Sob, sobitol; Gly3p, glyceraldeyde-3p; Ser, serine; Ala, alanine; Pyr, pyruvate; AceCoA, acetyl-CoA; Cit, citrate; Iso, isocitrate; Oxa, oxaloacetate;ADS, amorphadiene synthase.