EGFP-LC3 and tfLC3 diffuse more slowly than predicted by their molecular weights.

<p>COS-7 cells were transfected with the indicated constructs. The following day, confocal FRAP experiments were performed at 37°C as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009806#s4" target="_blank">Materials and Methods</a>. (<b>A</b>) Representative images collected during a FRAP experiment in COS-7 cells. Data are shown for measurements in the nucleus. Scale bar = 2 µm. (<b>B</b>) Recovery curves for EGFP (black circles), EGFP-LC3 (blue squares), and tfLC3 (red triangles) in the cytoplasm of COS-7 cells. Data show the mean values for 10 cells from a representative experiment. For clarity, error bars are not shown. (<b>C</b>) Recovery curves for EGFP (black circles), EGFP-LC3 (blue squares), and p53-GFP (green crosses) in the nucleus of COS-7 cells. Data show the mean ± SE for 10 cells from a representative experiment. For clarity, error bars are not shown. (<b>D</b>) Effective diffusion coefficients for EGFP, EGFP-LC3, tfLC3, and p53-GFP in the cytosol (white bars) and nucleus (gray bars) of COS-7 cells. Data show the mean ± SD for 30 cells from a total of 3 independent experiments. (<b>E</b>) Mobile fractions for EGFP, EGFP-LC3, tfLC3, and p53-GFP in the cytosol (white bars) and nucleus (gray bars) of COS-7 cells. Data show the mean ± SD for 30 cells from a total of 3 independent experiments.