The nucleocytoplasmic distribution of EGFP-LC3 is modestly affected by microtubule disruption.

<p>Cells were subjected to microtubule disruption with 5 µg/ml NZ or mock-treated (“control”) and fixed prior to imaging as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009806#s4" target="_blank">Materials and Methods</a>. (<b>A</b>) Effect of NZ on the distribution of EGFP-LC3 in COS-7 cells. (<b>B</b>) Quantification of N/C for soluble EGFP-LC3 in mock-treated or NZ-treated COS-7 cells. Data show the mean ± SD for 28–30 cells from 2 independent experiments. (<b>C</b>) Effect of NZ on the distribution of GFP-LC3 in HeLa cells. (<b>D</b>) As in B except data are for EGFP-LC3 in HeLa cells. Data show the mean ± SD for 62–72 cells from 2 independent experiments. Scale bar = 10 µm.