CTxB diffusion is confined by the actin cytoskeleton.

<p>(<b>A–D</b>) Subcellular distribution of (A) Alexa546-CTxB, (B) YFP-GL-GPI, (C) YFP-GT46 and (D) DiIC₁₆ in live COS-7 cells under control conditions or following LatA treatment as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034923#s4" target="_blank">Material and Methods</a>. A zoom of the boxed area in LatA treated cells is shown on the right for each marker. (<b>E</b>) Rhodamine phallodin staining in fixed COS-7 cells under control conditions or following LatA treatment. Bar, 10 µm. (<b>F</b>) COS-7 cells were treated with 1 µM LatA or mock-treated with 0.1% DMSO (“control”) for 5 minutes prior to imaging, and FRAP analysis was performed in the continued presence of LatA. Diffusion coefficients were measured for Alexa546-CTxB, YFP-GL-GPI, YFP-GT46 and DiIC₁₆ in control and LatA treated COS-7 cells at 37°C (mean ± SD for 13–23 cells). ** <i>p</i><0.01, Student t-test.