a<p>At the time of plating, the 168 and 168-pLK culture contained 1.3*10<sup>9</sup> viable cells/ml, the NCIB3610-pLK culture contained 0.9*10<sup>9</sup> viable cells/ml, and the JW49 contained 1.0*10<sup>9</sup> viable cells/ml. For transformations 6 µg chromosomal DNA from strain JWV184 per ml culture was used. - = not tested.</p
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
<p>Chemically competent <i>E</i>. <i>coli</i> DH5α and JM109 cells were transformed with 100pg μL<su...
a<p>Exponentially growing cells were supplied with isogenic chromosomal DNA at 1∶2.5 ratio (CFU:DNA)...
a<p>fold induction of competence compared to the control strain containing the empty pLOSS* vector a...
<p>Strain names: CAG, CAG18439; HB, HB101; and MC, MC4100. Cell-to-cell transformation (A) was perfo...
a<p>Results are mean of three replicates with standard deviation.</p>b<p>Chromosomal DNA from <i>C. ...
ml YPD medium at 30°C until early stationary phase (OD660 = 1.05-1.25) and then harvested by centrif...
<p><i>E</i>. <i>coli</i> DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV...
<p>Each strain possessed the <i>his3-Δ5′</i> substrate at the <i>LEU2</i> locus on chromosome III, a...
<p>LCC DNA ministring production efficiencies of Tel<sup>+</sup> (W3NN) cells for: 1) heat induced b...
<p>Effect of DNase I activity on lateral plasmid transfer in cell-mixed culture (A, B), and detectio...
a<p>The competent cells [BL21(DE3)RIL] used had efficiency of 1×10<sup>8</sup> transformants/µg supe...
<p><i>(</i>A) Sensitivity of a wild-type <i>U. muehlenbergii</i> strain to hygromycin B was evaluate...
Natural transformation, a mechanism that generates genetic diversity in Campylobacter jejuni, was st...
To research the effects of vector quantity and competence on the positive cloning rate, with a known...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
<p>Chemically competent <i>E</i>. <i>coli</i> DH5α and JM109 cells were transformed with 100pg μL<su...
a<p>Exponentially growing cells were supplied with isogenic chromosomal DNA at 1∶2.5 ratio (CFU:DNA)...
a<p>fold induction of competence compared to the control strain containing the empty pLOSS* vector a...
<p>Strain names: CAG, CAG18439; HB, HB101; and MC, MC4100. Cell-to-cell transformation (A) was perfo...
a<p>Results are mean of three replicates with standard deviation.</p>b<p>Chromosomal DNA from <i>C. ...
ml YPD medium at 30°C until early stationary phase (OD660 = 1.05-1.25) and then harvested by centrif...
<p><i>E</i>. <i>coli</i> DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV...
<p>Each strain possessed the <i>his3-Δ5′</i> substrate at the <i>LEU2</i> locus on chromosome III, a...
<p>LCC DNA ministring production efficiencies of Tel<sup>+</sup> (W3NN) cells for: 1) heat induced b...
<p>Effect of DNase I activity on lateral plasmid transfer in cell-mixed culture (A, B), and detectio...
a<p>The competent cells [BL21(DE3)RIL] used had efficiency of 1×10<sup>8</sup> transformants/µg supe...
<p><i>(</i>A) Sensitivity of a wild-type <i>U. muehlenbergii</i> strain to hygromycin B was evaluate...
Natural transformation, a mechanism that generates genetic diversity in Campylobacter jejuni, was st...
To research the effects of vector quantity and competence on the positive cloning rate, with a known...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
<p>Chemically competent <i>E</i>. <i>coli</i> DH5α and JM109 cells were transformed with 100pg μL<su...
a<p>Exponentially growing cells were supplied with isogenic chromosomal DNA at 1∶2.5 ratio (CFU:DNA)...