Increase in competence levels of tested strains using SMM culture medium.

a<p>fold induction of competence compared to the control strain containing the empty pLOSS* vector and grown with 200 µM IPTG.</p>b<p>In a typical experiment using SMM medium and 200 µM IPTG, the 168-pLK, NCIB 3610-pLK and JW49-pLK cultures contained ∼3.5*10⁸ viable cells/ml at the time of plating (OD₆₀₀ 0.65), and when using pHY300plk plasmid as donor DNA, approximately 40.0*10⁴ (168+ pLK) 3.35*10⁴ (NCIB3610 + pLK) and 0.47*10⁴ (JW49 + pLK) transformants/ml were obtained.</p><p>- = not tested.