Effect of culturing technique and volume on ministring production.

<p>LCC DNA ministring production efficiencies of Tel⁺ (W3NN) cells for: 1) heat induced batch cultivations across different culture volumes (10, 20, 50 ml) and heat induction duration (15, 30, 60 min); 2) heat induced two stage continuous cultivations (A & B); 3) 500 ml batch cultivation; and 4) non-induced controls. For 50 ml cultures, heat induction for 60 min led to production efficiencies significantly greater than heat induction for 30 min * (P<<0.01). Production efficiencies for two stage continuous (B) was significantly greater than two step continuous (A) ** (P<<0.01) and 500 ml batch cultivation ** (P<0.05). All heat induced cultures had production efficiencies significantly greater than non-induced samples *** (P<<0.01).