Implication of PGC-1α in GR transactivation.

<p>A/ Expression of PGC-1α. Total RNA was prepared from 158N cells. RT-PCR experiments were performed using primers recognizing specifically PGC-1α. PCR products were analyzed on agarose gel (2%) and visualized under UV. 18S RNA was detected by specific primers and used to normalize PGC-1α expression levels. B/ Implication of PGC-1α in the glucocorticoid pathway. 158N cells were transiently cotransfected with either 0.2 µg of (GRE)2-TATA-Luc, 0.1 µg of pRSV-βGal plasmids and increasing amounts of PGC-1α expression vector, as indicated. Eighteen hours after transfection, cells were incubated with Dex (10⁻⁶ M) for 24 h, and then luciferase and β-galactosidase activities were analyzed. Results are expressed as percentage of the basal activity and represent the mean +/− SEM of at least four independent experiments performed in duplicate. **p<0.01 when compared between cells transfected with PGC-1α using Bonferroni's test after ANOVA.