Knock-down of PGC-1α accelerated high glucose-induced VSMC proliferation and migration.

<p>VSMCs transfected with siRNA (SI) or the negative control (N), were either left untreated or stimulated with 15 mM gluoose (HG). The interference effect was assessed by quantitative PCR analysis and data were shown as the ratio of PGC-1α/β-actin (A), **P<0.01compared with the negative control group (N). 120 µg total proteins from VSMCs were assayed for Western blot with PGC-1 antibody to confirm the interference effiency (B). Effects of decreased PGC-1α on high glucose-induced VSMC proliferation were determined by cell counting (C). VSMC migration was determined by wound healing (D) and transwell analysis (E). Individual data points in this figure represents the mean±S.E.M (n = 6). *P<0.05, #P<0.001 compared with the control group (N+HG).