<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007475#pone-0007475-g001" target="_blank">Figure 1</a>. Normal human dermal fibroblasts and HUVECs were cultured for 2 days in 8-well glass slides. Cells were then air-dried, fixed, and stained with antibodies against A–C) CD43, D–F) CD44, G–I) CD45, J–L) LSP-1, and M–O) CD29. Cells were then counterstained with hematoxylin to identify nuclei. Bar is 50 µm.</p
<p><b>A</b>. Representative flow cytometry histograms show staining of cultured RASFib (Third passag...
<p>Fibroblast cell lines, designated CoDa, were established from the autopsy skin tissues. Panel a s...
<p>SF from RA, OA and normal synovial tissues, and normal skin dermal fibroblasts (DF) were cultured...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>PBMC were cultured in SFM at 5×10<sup>5</sup> cells/ml in 8-well glass slides for 21 days. Cells ...
<p>PBMC were cultured in SFM at 5×10<sup>5</sup> cells/ml in 8-well glass slides for 1 hour (<i>ex v...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>PBMC were cultured in 8-well glass slides in SFM for 7 days, in the presence or absence of IL-4, ...
<p>PBMCs were cultured in Fibrolife medium for five days in the presence or absence of 25 mM additio...
<p>PBMCs were stimulated with anti-CD3/CH-296. On day 10, cultured cells were harvested for transfus...
<p>“HOPE-fixed” human lung tissue sections were stained with antibodies against CD32, CD45RO, CD68, ...
<p>Dermal fibroblasts (1×10<sup>6</sup>) were seeded in 6-well plate pre-coated with SR or three C-S...
<p>Immunocytology showing CD31 (green) and PROX1 (magenta). Nuclei are counter-stained with DAPI (bl...
<p>Human A-C) lung fibroblasts, D-F) lung fibroblasts incubated with 50 ng/ml TNF-α, or G-I) lung ep...
<p>CD34<sup>+</sup> cells (1×10<sup>4</sup>/ml) purified by immunomagnetic positive selection were c...
<p><b>A</b>. Representative flow cytometry histograms show staining of cultured RASFib (Third passag...
<p>Fibroblast cell lines, designated CoDa, were established from the autopsy skin tissues. Panel a s...
<p>SF from RA, OA and normal synovial tissues, and normal skin dermal fibroblasts (DF) were cultured...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>PBMC were cultured in SFM at 5×10<sup>5</sup> cells/ml in 8-well glass slides for 21 days. Cells ...
<p>PBMC were cultured in SFM at 5×10<sup>5</sup> cells/ml in 8-well glass slides for 1 hour (<i>ex v...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>PBMC were cultured in 8-well glass slides in SFM for 7 days, in the presence or absence of IL-4, ...
<p>PBMCs were cultured in Fibrolife medium for five days in the presence or absence of 25 mM additio...
<p>PBMCs were stimulated with anti-CD3/CH-296. On day 10, cultured cells were harvested for transfus...
<p>“HOPE-fixed” human lung tissue sections were stained with antibodies against CD32, CD45RO, CD68, ...
<p>Dermal fibroblasts (1×10<sup>6</sup>) were seeded in 6-well plate pre-coated with SR or three C-S...
<p>Immunocytology showing CD31 (green) and PROX1 (magenta). Nuclei are counter-stained with DAPI (bl...
<p>Human A-C) lung fibroblasts, D-F) lung fibroblasts incubated with 50 ng/ml TNF-α, or G-I) lung ep...
<p>CD34<sup>+</sup> cells (1×10<sup>4</sup>/ml) purified by immunomagnetic positive selection were c...
<p><b>A</b>. Representative flow cytometry histograms show staining of cultured RASFib (Third passag...
<p>Fibroblast cell lines, designated CoDa, were established from the autopsy skin tissues. Panel a s...
<p>SF from RA, OA and normal synovial tissues, and normal skin dermal fibroblasts (DF) were cultured...