Human dermal fibroblasts cultured on C-SR grow faster and showed a more fibroblastic appearance.

<p>Dermal fibroblasts (1×10⁶) were seeded in 6-well plate pre-coated with SR or three C-SRs (C-SR-1, C-SR-2 and C-SR-3). The cells were incubated in 37°C incubator for 48 hours for observation of cell morphology. (A) FITC-labeled actin tracer was used to observe cytoskeleton by immuno-fluorescence microscopy. Fibroblasts cultured on C-SRs had more and larger filopodia spreading out, and their microfilament stretched longer and arranged more regularly. (B) SEM images further demonstrated a more fibroblastic appearance of fibroblasts cultured on C-SRs. (C) Cell area depicted higher values for cells on C-SRs than those on SR. But the difference was not statistically significant between any two groups (p>0.05). (D) Dermal fibroblasts were cultured in 96-well plate (at a density 1×10³ cells/well) for 24, 48 and 96 hours. The cell viability was analyzed by CCK-8 assay. The cell viability was also increased by C ion implantation. *p<0.05 The difference was statistically significant when the substrate was compared to SR at any time point. ^#p<0.05 The difference was statistically significant when the substrate was compared to TCP at any time point. Experiments in this figure were repeated three times, similar results were obtained each time. Bar = 20 µm. All data are expressed as mean ± SD (error bars).