<p>SF from RA, OA and normal synovial tissues, and normal skin dermal fibroblasts (DF) were cultured on glass coverslips and immunolabeled (red) for gp38 expression. TNF-α treated DF cultures are also shown (DAPI nuclear counterstaining). Flow cytometric detection of surface gp38 by in RA SF and DF untreated (basal) or treated with TNF-α for 24h. Data are representative of 6 SF and 3 DF lines (*p = 0.03 basal vs TNF-α treated). MFI: Mean fluorescence intensity.</p
Fibroblast-like cells were obtained from a nodule of a patient with fibroblastic rheumatism, and gro...
<p>CD55, CD46, and CD59 expression was measured by flow cytometry on cultured FLS from patients with...
<p>Dermal fibroblasts (1×10<sup>6</sup>) were seeded in 6-well plate pre-coated with SR or three C-S...
Abstract The study of the pathogenetic mechanisms of rheumatic diseases is in general carried out th...
<p><b>A</b>. Representative flow cytometry histograms show staining of cultured RASFib (Third passag...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>(A) Experimental design. Synovial MSCs were cultured with or without TNFα (Control) for 14 days f...
<p><b>(A)</b> IHC immunoperoxidase labelling of gp38 expression in normal, OA and RA tissues. RA CTR...
Abstract Fibroblast-like synoviocytes (FLS) play an important pathological role in persistent inflam...
<p>FACS plot of PD1 expression on gated live CD45<sup>+</sup> lymphocytes (A), CD4<sup>+</sup> (B) a...
<p>(A and B) Typical immunofluorescence microscopy images of DAPI, HIF-2α, and FLS markers (vimentin...
<p><b>A</b>. MNCs from PB and SF of JIA patients were polyclonally stimulated with PMA plus ionomyci...
<p><b>A:</b> Fibroblasts grown in a monolayer were serum-starved for 24 h before the stimulation of ...
<p><b>(A)</b> After RA synovial fibroblasts were cultured with 0–50 ng/ml of VEGF for 72 h, the RANK...
RA is a chronic inflammatory disease that is challenging to model in vitro. Historically cells have...
Fibroblast-like cells were obtained from a nodule of a patient with fibroblastic rheumatism, and gro...
<p>CD55, CD46, and CD59 expression was measured by flow cytometry on cultured FLS from patients with...
<p>Dermal fibroblasts (1×10<sup>6</sup>) were seeded in 6-well plate pre-coated with SR or three C-S...
Abstract The study of the pathogenetic mechanisms of rheumatic diseases is in general carried out th...
<p><b>A</b>. Representative flow cytometry histograms show staining of cultured RASFib (Third passag...
<p>PBMC were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journ...
<p>(A) Experimental design. Synovial MSCs were cultured with or without TNFα (Control) for 14 days f...
<p><b>(A)</b> IHC immunoperoxidase labelling of gp38 expression in normal, OA and RA tissues. RA CTR...
Abstract Fibroblast-like synoviocytes (FLS) play an important pathological role in persistent inflam...
<p>FACS plot of PD1 expression on gated live CD45<sup>+</sup> lymphocytes (A), CD4<sup>+</sup> (B) a...
<p>(A and B) Typical immunofluorescence microscopy images of DAPI, HIF-2α, and FLS markers (vimentin...
<p><b>A</b>. MNCs from PB and SF of JIA patients were polyclonally stimulated with PMA plus ionomyci...
<p><b>A:</b> Fibroblasts grown in a monolayer were serum-starved for 24 h before the stimulation of ...
<p><b>(A)</b> After RA synovial fibroblasts were cultured with 0–50 ng/ml of VEGF for 72 h, the RANK...
RA is a chronic inflammatory disease that is challenging to model in vitro. Historically cells have...
Fibroblast-like cells were obtained from a nodule of a patient with fibroblastic rheumatism, and gro...
<p>CD55, CD46, and CD59 expression was measured by flow cytometry on cultured FLS from patients with...
<p>Dermal fibroblasts (1×10<sup>6</sup>) were seeded in 6-well plate pre-coated with SR or three C-S...