Deletion of 3′ end IRF2 UTR RNA showed reduced activity:

<p>(A) Schematic representation of bicistronic construct used for translation studies. (B) Bicistronic plasmid (1 µg) either pRΔEIRF2F or pRΔE3′δ25IRF2F were transiently transfected into HeLa cells. 16 hrs post transfection, respective luciferase activities corresponding to Fluc and RLuc were measured and shown separately. Transfection efficiencies were normalized by co-transfecting with a β-galactosidase plasmid. The data represents mean±s.d. from three independent experiments.