Comparison of IRES activities following DNA or RNA transfection.

<p>(A, C, E) C2C12 cells were transfected with bicistronic plasmids containing either the FGF1 IRES, or EMCV IRES or a hairpin (control without IRES) and with siRNA siM, sip54 or sic, and IRES activities measured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136466#pone.0136466.g004" target="_blank">Fig 4B, 4D and 4F</a> C2C12 cells were transfected with siRNA siM, sip54 or sic and 24h later with bicistronic mRNAs containing either the FGF1 IRES, or the EMCV IRES, or a hairpin as above. mRNAs were transcribed in vitro, capped and polyadenylated, as described in Mat. & Meth. The LucF/LucR activity ratio was measured 12h post-transfection. Experiments were performed in biological triplicates and repeated three times. The Student test was used. A representative experiment is shown (mean +- standard deviation, *p<0.05, **p<0.01, ***<0.001). For A and B, the Luc R and Luc F values are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136466#pone.0136466.s004" target="_blank">S4 File</a>.