Temperature-dependent translation initiation by viral IRES elements.

<p><b>(A)</b> Schematic representation of the bicistronic reporter constructs. NIH3T3 cells were transfected with bicistronic reporter constructs containing an IRES element from EMCV, FMDV, HCV, HRV or PV, or a control pRF construct. 24 hours post-transfection, cells were cultured at 30°C <b>(B)</b>, 35°C <b>(C)</b>, or 37°C for 8 hours. Firefly and <i>Renilla</i> luciferase units were measured using the Dual-Luciferase Reporter Assay System. Firefly/Renilla represents the ratio of viral IRES-mediated translation to cap-dependent translation. Data are expressed as the mean ± SE of 3 independent samples. Statistical analysis was conducted using two-way ANOVA and multiple comparison test. *p<0.01.