Two-electrode voltage clamp electrophysiological experiments on <i>Xenopus</i> oocytes.

<p>A) Oocytes injected with <i>Asu- unc-38</i> or <i>Asu-unc-29</i> cRNAs alone produced no functional receptors, whereas oocytes injected with both subunit cRNAs in an equimolar ratio gave robust responses to application of 100 µM acetylcholine (ACh). B) Oocytes injected with an equimolar ratio of <i>Asu-unc-38</i> and <i>Asu-unc-29</i> cRNA responded to 100 µM acetylcholine (ACh), 100 µM levamisole (lev) and 100 µM nicotine (nic). These responses could all be reversibly blocked by application of 10 µM mecamylamine. C) Dose-response relationships for the agonists levamisole (lev, grey circles), acetylcholine (ACh, black diamonds) and nicotine (nic, black triangles) from oocytes injected with an equimolar ratio of <i>Asu-unc-38</i> and <i>Asu-unc-29</i>. Responses are all normalized to the response to 100 µM acetylcholine. N = 3 (where N is batches of oocytes) and n = 6 (where n is the number of individual oocytes) minimum for each data point.