Electrophysiological properties of poP2RX7 expressed in <i>Xenopus</i> oocytes.

<p>A and B. Representative recordings of individual oocytes expressing poP2RX7 (A) or rP2RX7 (B), currents were gated with 1 mM ATP dissolved in Barth's or low-divalent (LD) media. (C). Summary of the peak amplitudes obtained in Barth's or LD media in oocytes expressing poP2RX7 or rP2RX7. D and E. Currents evoked by 1 mM ATP and 100 µM BzATP from individual oocytes expressing poP2RX7 (D) or rP2RX7 (E), in LD media. (F). Concentration-response curves for BzATP (open squares) and ATP (black circles) for poP2RX7 (left graph) or rP2RX7 (right graph). (G). Current facilitation of poP2RX7 (left recordings) and rP2RX7 (right recordings). In each case, three consecutive ATP pulses were applied to the same oocyte. (H). Representative recording of poP2RX7-expressing oocytes showing the currents evoked by ATP alone and the inhibition induced by pre-application with BBG for 2 min followed by an co-application with 1 µM BBG (left recordings) or 20 µM AZ10606120 (right recordings). (I). Summary of the inhibition induced by BBG or AZ10606120 (AZ) at poP2RX7 (open bars) and rP2RX7 (black bars). *<i>p</i><0.05, compared with rP2RX7 by Mann-Whitney test, n = 3–5.