Overexpression of <i>SCC1</i> or <i>SCC2</i> suppress the synthetic lethality between <i>elg1</i> and <i>ctf4</i>.

<p>A <i>elg1 ctf4</i> double mutant maintained alive by the presence of a <i>URA3</i>-marked plasmid carrying the <i>ELG1</i> gene was transformed with <i>LEU2</i>-marked plasmids overexpressing <i>SCC1, SCC2, SMC1</i> or <i>SMC3,</i> or, as controls, with an empty vector or a plasmid carrying <i>ELG1</i>. Cells were successively diluted ten-fold and plated on plates lacking Uracil (SD-Ura), Leucine (SD-Leu), or on plates containing 5-fluoroorotic acid (5-FOA), which select for cells that became Ura- (i.e., lost the <i>URA3</i>-marked, <i>ELG1</i>-containing plasmid). Only plasmids overexpressing <i>SCC1</i> and <i>SCC2</i> allowed cells to grow on 5-FOA plates.