Cross-dependencies between different genes for the ability to confer resistance to 5-FU when overexpressed.

<p>Each plasmid was transformed into yeast knockout strains where one of the other resistance genes had been deleted. Overexpression plasmids (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196840#pone.0196840.g001" target="_blank">Fig 1</a>) are shown at the top, yeast strains at the left, and drug concentrations at the bottom in the figure. Transformants were grown in liquid medium to late exponential phase, diluted, and then spotted onto uracil-less SC glucose (Glu-U) plates with or without 4 μg/ml 5-FU. Also shown to the right are results obtained on uracil-less SC galactose (Gal-U) plates for the <i>LOG1</i> gene and the pHR81 vector control. The + sign stands for the haploid wild type control strain BY4742, +/+ stands for the diploid wild type control strain BY4743, and +/<i>dut1</i> for the diploid <i>DUT1/dut1</i> heterozygote. The control vector is pHR81 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196840#pone.0196840.ref030" target="_blank">30</a>]. The no drug control plate was photographed after 3 days and the 5-FU plate after 5 days.