RT-PCR analysis of mRNA expression in GH4-hα7 cells after 24 hr exposure to peptides.

<p>GAPDH expression was used as an internal standard. Gels shown are representative results of experiments; accompanying graphs provide semi-quantitative data determined from a minimum of 3 separate experiments. Asterisks (*) indicate values statistically different from controls. A. Effect of varying concentrations of AChE peptides T14 and T30 on α7-nAChR expression. B. Effects of 100 nM control peptides, 10 nM full-length T-AChE, or 10 nM truncated T-AChE on α7-nAChR expression. Lane 1 = Control, 2 = T15, 3 = S14, 4 = B14, 5 = SB14, 6 = full-length T-AChE, 7 = truncated T-AChE (T548). C. Effects of MLA (10 µM) on peptide-induced changes in α7-nAChR expression in cells treated with 100 nM T14, T30, or T15. Hash marks (#) indicate peptide+MLA values significantly different from peptide alone values.