Viral DNA replication and growth defects in the absence of lamin A/C.

<p>A) Kinetics of viral DNA replication in <i>Lmna^+/+</i> and <i>Lmna^−/−</i> cells. DNA was extracted from whole cell lysate of mock- or HSV-infected <i>Lmna^+/+</i> and <i>Lmna^−/−</i> MEFs at the times indicated and quantified by real-time PCR. Relative viral DNA levels, as calculated in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000071#s4" target="_blank">Materials and Methods</a>, are shown. The error bars represent the standard deviation from three experiments. B) HSV growth curve at high MOI. <i>Lmna^+/+</i> and <i>Lmna^−/−</i> MEFs were infected with HSV at an MOI of 10. At the times indicated, cells and supernatant were harvested, and total infectious virus was measured by plaque assay on Vero cells. The results shown were from triplicate cultures. C) HSV growth curve at low MOI. <i>Lmna^+/+</i> and <i>Lmna^−/−</i> MEFs were infected with HSV at an MOI of 0.1. At the times indicated, cells and supernatant were harvested and titered on Vero cells, as described above. The results shown were from triplicate cultures.