HSV-1 DNA replication does not decrease H3.1 mobilization in U2OS cells.

<p>(<b>A</b>) Average normalized levels of free GFP-H3.1 relative to untreated mock-infected cells. U2OS cells were transfected with plasmids expressing GFP-H3.1. Transfected cells were mock-infected or infected with 6 PFU/cell of strain KOS in the presence of 400 µg/ml PAA (<b>PAA KOS</b>) or no drug (<b>KOS</b>). Mobilization of GFP-H3.1 was examined from 4 to 5 (<b>4 hpi</b>) or 7 to 8 (<b>7 hpi</b>) hpi by FRAP; error bars, SEM; dashed line, average normalized level of free GFP-H3.1 in mock-infected cells. (<b>B</b>) Average initial rates of normalized fluorescence recovery of GFP-H3.1 relative to untreated mock-infected cells; error bars, SEM; dashed line, the average initial rate of normalized fluorescence recovery in mock-infected cells. KOS data re-plotted from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003695#ppat-1003695-g008" target="_blank">Figure 8</a> for comparison. **, P<0.01; <i>n.s.</i>, not significant.