The effect of resveratrol on cell viability, in vitro wound closure, and cell migration and invasion in Huh7 cells.

<p>(A) Huh7 cells were treated with resveratrol (0, 25, 50, 75, 100 and 200 µM) for 24 h and 48 h before being subjected to a MTT assay that tested cell viability. The values represented the means ± SD of at least 3 independent experiments. (B) Normal hepatocytes were treated with resveratrol (0, 25, 50, 75, 100 and 200 µM) for 24 h before being subjected to a MTT assay that tested cell viability. The values represented the means ± SD of at least 3 independent experiments. (C) Huh7 cells were wounded and then treated with vehicle (DMSO) or resveratrol (25, 50, 75, and 100 µM) for 0, 12, and 24 h in a 0.5% FBS-containing medium. At 0, 12, and 24 h, the phase-contrast pictures of the wounds at 3 locations were taken. (D & E) Cell migration and invasion were measured using a Boyden chamber for 16 and 24 h with polycarbonate filters, respectively. The migration and invasion abilities of Huh7 cells were quantified by counting the number of cells that invaded the underside of the porous polycarbonate, as described in the Materials and Methods section. The values represented the means ± SD of at least 3 independent experiments. <i>*p<</i>0.05, compared with the vehicle group.