Consequence of the SOD2 Knockdown

<div><p>(A) Histochemical staining reveals that SDH activity in the heart of transgenic line-8 mice was reduced compared with the wild-type littermates (B).</p><p>(C) ROS levels are increased in fibroblasts from the skeletal muscles of transgenic line-26 (top panel) and line-8 (bottom panel) mice, compared with those from the wild-type mice (middle panel). AU, arbitrary units.</p><p>(D) Fibroblasts from the transgenic line-8 mice have elevated sensitivity to oxidative stress compared with those from the wild-type mice, and this sensitivity can be corrected by expressing an RNAi-resistant SOD2 (line 8 + SOD2r). The data are means observed in cells isolated from four individual mice. Error bars are SEM. The asterisks indicate significant difference as compared to either WT or rescued cells (<i>p</i> < 0.05).</p><p>(E) Western blot detects SOD2 protein levels in fibroblasts isolated from the skeletal muscle of the wild-type and transgenic line-8 mice. The third lane is from the line-8 cells transduced with RAd expressing the siRNA-resistant SOD2.</p>