Unsuccessful Attempts of Liver Infection

<div><p>(A) The composite image of ten selected frames from an intravital movie shows a paralyzed or dead P. berghei sporozoite that is eventually dislodged and flushed out of the liver lobule (short arrows). The parasite maintains a fixed crescent shape, fails to cling to the sinusoidal cell layer, and makes no attempt to glide against the bloodstream. The long arrow (dotted line) indicates the direction of the blood flow.</p> <p>(B) Projection composed of 14 selected frames extracted from an intravital movie showing a GFP P. berghei sporozoite that initially transmigrates in the liver parenchyma (dotted lines), but then reenters a sinusoid (arrow) and is swept away with the bloodstream (solid lines).</p> <p>Bars = 10 μm. See <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030192#sv009" target="_blank">Videos S9</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030192#sv010" target="_blank">S10</a>.</p>