Lipid MALDI ion images reveal differences in PC zonation across the liver lobule.

<p>Representative MALDI IMS images of selected liver specimens obtained from obese subjects with normal liver histology (<b>A–D</b>), SS (<b>E–H</b>), and NASH (<b>I–L</b>). (<b>A, E, I</b>) Photomicrographs of H&E stained sections from each specimen (far left column) indicate lobular zones (zone 1 - Z1; zone 3 - Z3). Comparison of selected PCs by disease state: MALDI images of <i>m/</i>z 796.52 PC 34∶2 [M+K]⁺ in obese normal (<b>B</b>), SS (<b>F</b>), and NASH (<b>J</b>) specimens. MALDI images of <i>m/</i>z 820.52 PC 36∶4 [M+K]⁺ in obese normal (<b>C</b>), SS (<b>G</b>), and NASH (<b>K</b>) specimens. MALDI image of <i>m/</i>z 782.54 PC 34∶1 [M+K]⁺ in obese normal (<b>D</b>), SS (<b>H</b>), and NASH (<b>L)</b> specimens. Ion intensity color scale for all ion images is shown at the top of panels B and C. Scale bar = 500 µm.