Diagram depicting loss of phospholipid zonation in NASH.

<p>(<b>A</b>) In obese normal liver tissue the PCs with 34 and 36 carbons, were most abundant and localized to zones 2 (Z2) and 3 (Z3). PC 32∶0 localized exclusively to portal tracts in all specimens. PEMT localized exclusively to zone 3 hepatocytes. (<b>B</b>) In SS specimens, there were increased amounts of PC 34 and 36 carbon PCs in zone 3 and zone 2 but a decrease in their abundance in zone 1. (<b>C</b>) In NASH, the zonation of most lipid molecular species was reduced with few exceptions. In both SS and NASH, PEMT expressions were panlobular, but with NASH, additional staining was observed at sites of inflammation.