Destabilization of pThoc1 by treatment with DRB is dependent on <i>NEDD4-1</i>.

<p>A) HeLa cells engineered to express tetracycline inducible shRNA targeting <i>NEDD4-1</i> were treated with tetracycline and/or cycloheximide as indicated and cells extracted at the indicated times post cycloheximide treatment. The indicated proteins were analyzed by western blotting. β-actin serves as a loading control. B) Wild type of NEDD4-1 null MEFs were treated with DRB and cycloheximide and extracted at the indicated times post-treatment. <i>Thoc1</i> protein levels were assayed by western blotting with β-actin serving as a loading control. C) The western blot in B) was analyzed by densitometry and the optical density of the pThoc1 band relative to the actin band plotted. pThoc1 stability is increased in the absence of <i>NEDD4-1</i>.