TEM4 and RhoC are required to suppress myosin contractility.

<p>Knockdown of TEM4 or RhoC impairs cellular ability to down regulate myosin contractility. Cells depleted of TEM4 or RhoC or NS control were left untreated (GM), treated with nocodazole (Noc) or treated with nocodazole with subsequent nocodazole washout. Phosphorylation of MLC2 was determined by western blot analysis of whole cell lysates (A), quantitated using densitometry and graphed (B). Phospho-MLC2 levels in each group were normalized to levels in the untreated NS control cells that were set to 1. Data are mean ± s.e.m. measured in three independent experiments.