<p>HEK 293T cells were transfected with AMOTL1, Nedd4.2 and YAP1, as indicated. Cells were treated with 5 µM MG132 for 120 min. Lysates were analyzed by Western blotting. AMOTL1, Nedd4.2 and YAP1 were detected with anti-Flag, anti-myc and anti-V5 antibodies, respectively. Actin was used as a loading control.</p
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(<b>A</b>) Typical profile of proteins purified on anti-FLAG M2 affinity gel from PC12TetOn cells...
poster abstractAmot adaptor proteins bind and integrate signaling that controls cell po-larity and g...
<p>HEK293T cells were transiently transfected with either YAP1 or Nedd4.2 shRNA. Even a small reduct...
<p><i>A</i>, Nedd4.2 reduces AMOTL1 protein levels. Co-expression of myc-tagged Nedd4.2 (myc.Nedd4.2...
<p><i>A</i>, Nedd4.2 facilitates the ubiquitylation of AMOTL1. HEK293T cells were transfected with A...
<p><i>A</i>, Flag-tagged c-Abl (F.cAbl), immobilized on M2 sepharose beads, precipitates Nedd4.2. Im...
<p><i>A</i>, Myc-Nedd4.2 proteins purified from HEK293T cells, co-expressing either wild type or kin...
<p><i>A</i>, The two PPxY motifs of AMOTL1 (PY1: PPEY, amino acid 310–313, and PY2: PPEY, amino acid...
<p>A-C) HEK293T cells were co-transfected with plasmids expressing myc-hIFITM3 or FLAG-NEDD4 as indi...
The HECT E3 ubiquitin ligase Nedd4-1 was previously shown to regulate diverse processes such as cell...
<p>(<b>A</b>) HEK293 cells were transfected with MAK-V-FLAG expression plasmid (<i>MAK-V-FLAG +</i>)...
<p>A) 293 cell extracts were immunoprecipitated with a pThoc1 specific antibody or non-specific IgG,...
<p>A) HeLa cells were transfected with the <i>THOC1</i> and His-Ub expression vectors (all lanes). I...
<p>293 cells were transfected with HXB2 and a plasmid encoding either rat Nedd4 (rNedd4) or a rat Ne...
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(<b>A</b>) Typical profile of proteins purified on anti-FLAG M2 affinity gel from PC12TetOn cells...
poster abstractAmot adaptor proteins bind and integrate signaling that controls cell po-larity and g...
<p>HEK293T cells were transiently transfected with either YAP1 or Nedd4.2 shRNA. Even a small reduct...
<p><i>A</i>, Nedd4.2 reduces AMOTL1 protein levels. Co-expression of myc-tagged Nedd4.2 (myc.Nedd4.2...
<p><i>A</i>, Nedd4.2 facilitates the ubiquitylation of AMOTL1. HEK293T cells were transfected with A...
<p><i>A</i>, Flag-tagged c-Abl (F.cAbl), immobilized on M2 sepharose beads, precipitates Nedd4.2. Im...
<p><i>A</i>, Myc-Nedd4.2 proteins purified from HEK293T cells, co-expressing either wild type or kin...
<p><i>A</i>, The two PPxY motifs of AMOTL1 (PY1: PPEY, amino acid 310–313, and PY2: PPEY, amino acid...
<p>A-C) HEK293T cells were co-transfected with plasmids expressing myc-hIFITM3 or FLAG-NEDD4 as indi...
The HECT E3 ubiquitin ligase Nedd4-1 was previously shown to regulate diverse processes such as cell...
<p>(<b>A</b>) HEK293 cells were transfected with MAK-V-FLAG expression plasmid (<i>MAK-V-FLAG +</i>)...
<p>A) 293 cell extracts were immunoprecipitated with a pThoc1 specific antibody or non-specific IgG,...
<p>A) HeLa cells were transfected with the <i>THOC1</i> and His-Ub expression vectors (all lanes). I...
<p>293 cells were transfected with HXB2 and a plasmid encoding either rat Nedd4 (rNedd4) or a rat Ne...
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(<b>A</b>) Typical profile of proteins purified on anti-FLAG M2 affinity gel from PC12TetOn cells...
poster abstractAmot adaptor proteins bind and integrate signaling that controls cell po-larity and g...
DOI
Add to ORKG