T_reg cells induce a suppressive DC phenotype.

<p>BALB/c BMDC were left untreated or stimulated with LPS (100 ng/ml) and cocultured with C57BL/6 T_reg cells in an 1∶1 ratio (DC _sup). For optimal T_reg stimulation, a soluble anti-CD3-mAb (3 µg/ml) was added. After 4 h expression of CD80, B7-H1 and B7-DC was determined by flow cytometry gating on CD11c⁺ MHCII⁺ cells. All depicted results were assayed in triplicate wells and are representative of three independent experiments. (*) indicates significant differences by Mann-Whitney test; n.s. – no significant differences.