Characteristic profile of tDCs derived from D1 mice.

<p>iDCs, mDCs, tDCs and mtDCs were induced in vitro as described in the Materials and Methods section, following which CD11c+ cells were harvested. (<b>A</b>) These four types of DC were stained with IA-IE, CD80, CD86, CD40 (thick lines) respectively or isotype-matched mAbs (thin lines) and the expression of those markers was analyzed by FACS flow cytometry. The frequency of positively stained cells and MFI of a representative of 10 independent experiments are shown in the FACS profile. (<b>B</b>) Expression of IL-12p40, IL-10, TGF-β, FasL, IDO and arginase by DC were determined by real-time PCR. All results were normalized to the expression of the housekeeping gene β-actin, and expressed as the mean ± SD of six independent experiments. *P <0.05 in comparisons of iDCs vs. tDCs, or mDCs vs. mtDCs by unpaired t-tests.