<p>Initial rates of phosphate release (<i>vi</i>) were obtained in the absence (♦) and in the presence of poly(C): 0.50 M () or 3.1 M (). ATP concentration was 0.10 mM and reactions were carried out in the same reaction media as were indicated above (see Materials and Methods). Data showed in (a) as a function of [NS3h] are plotted in (b) as a function of the ratio [poly(C)]/[NS3h].</p
<p>Effect of AMP, ADP and ATP on RTPase activity: CHIKV-nsP2T was incubated with 5′-[γ-<sup>32</sup>...
<p>(A) Rates of ATPase activity of MtbRho and EcRho in presence of different RNAs. MtbRho is a weake...
<p>Semi-log plots of the steady-state ATP dependence of the normalized concentrations of (i) one-nuc...
<p>Initial rates of phosphate release (<i>vi</i>) are plotted as a function of [poly(C)] (a) or [pol...
<p>Initial rates of ATP hydrolysis were obtained at different concentrations of poly(A) (a) or poly(...
<p>Effect of poly (U) RNA on ATPase activity: CHIKV-nsP2T protein was incubated in a 50 µl reaction ...
<p>PolyP PAGE was performed in 24% polyacrylamide gel with 7M urea; toluidine blue staining. PolyP<s...
<p>(<i>A</i>) Samples (5 µg) of the dialyzed nickel-agarose fractions of wt Rig-I (1–925), Rig-I-ΔRD...
<p><b>(A)</b> The standard RNA helix (0.1 pmol) was reacted with MBP-fusion 2C<sup>ATPase</sup> Wt (...
Kinetics of a self-capping RNA, Iso6, have been investigated to constrain the catalytic mechanism. T...
<p><b>(A)</b> Schematic illustration of the standard RNA helix substrate that contains both 3′- and ...
<p>(A) Effect of an RNA trap on NS3 unwinding activity. Representative gels showing time courses of ...
dependent RNA polymerase was studied during in vitro transcription reactions. In complete trans-crip...
<p>(A) The reaction mixtures were incubated with [γ-<sup>32</sup>P] ATP as described in <a href="htt...
<p>The increase in reaction rates (expressed as specific activities v/e (s<sup>-1</sup>)) indicates ...
<p>Effect of AMP, ADP and ATP on RTPase activity: CHIKV-nsP2T was incubated with 5′-[γ-<sup>32</sup>...
<p>(A) Rates of ATPase activity of MtbRho and EcRho in presence of different RNAs. MtbRho is a weake...
<p>Semi-log plots of the steady-state ATP dependence of the normalized concentrations of (i) one-nuc...
<p>Initial rates of phosphate release (<i>vi</i>) are plotted as a function of [poly(C)] (a) or [pol...
<p>Initial rates of ATP hydrolysis were obtained at different concentrations of poly(A) (a) or poly(...
<p>Effect of poly (U) RNA on ATPase activity: CHIKV-nsP2T protein was incubated in a 50 µl reaction ...
<p>PolyP PAGE was performed in 24% polyacrylamide gel with 7M urea; toluidine blue staining. PolyP<s...
<p>(<i>A</i>) Samples (5 µg) of the dialyzed nickel-agarose fractions of wt Rig-I (1–925), Rig-I-ΔRD...
<p><b>(A)</b> The standard RNA helix (0.1 pmol) was reacted with MBP-fusion 2C<sup>ATPase</sup> Wt (...
Kinetics of a self-capping RNA, Iso6, have been investigated to constrain the catalytic mechanism. T...
<p><b>(A)</b> Schematic illustration of the standard RNA helix substrate that contains both 3′- and ...
<p>(A) Effect of an RNA trap on NS3 unwinding activity. Representative gels showing time courses of ...
dependent RNA polymerase was studied during in vitro transcription reactions. In complete trans-crip...
<p>(A) The reaction mixtures were incubated with [γ-<sup>32</sup>P] ATP as described in <a href="htt...
<p>The increase in reaction rates (expressed as specific activities v/e (s<sup>-1</sup>)) indicates ...
<p>Effect of AMP, ADP and ATP on RTPase activity: CHIKV-nsP2T was incubated with 5′-[γ-<sup>32</sup>...
<p>(A) Rates of ATPase activity of MtbRho and EcRho in presence of different RNAs. MtbRho is a weake...
<p>Semi-log plots of the steady-state ATP dependence of the normalized concentrations of (i) one-nuc...