RNA preference by MtbRho.

<p>(A) Rates of ATPase activity of MtbRho and EcRho in presence of different RNAs. MtbRho is a weaker ATPase when polyC)is used(grey bars). But, in presence of mycobacterial RNA (brown bars), they have comparable rates of hydrolysis, while only MtbRho can hydrolyse ATP in presence of <i>sdaA</i> RNA(red bars). (B) Differential NTP hydrolysis by MtbRho. MtbRho can hydrolyze ATP and GTP in presence of the mycobacterial total RNA and <i>sdaA</i> RNA. Colorimetric assay was carried out as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107474#s4" target="_blank">Methods</a> (C) Differential ability of MtbRho to bind ATP and CTP. γ-³²P-ATP alone can be UV-crosslinked to MtbRho (lane 1), and visualized on 8% SDS-PAGE. Addition of unlabeled ATP to the reaction competes out the γ-³²P-ATP (lanes 2–4), but unlabeled CTP fails to do so (lanes 5–8). The reaction conditions were similar to ATPase assay, but without the addition of RNA.