Effect of C-29EA on ATPase and RNA-binding activities of NS3 helicase.

<p>(A) The reaction mixtures were incubated with [γ-³²P] ATP as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048685#s4" target="_blank">Materials and Methods</a>. The reaction mixtures were subjected to thin-layer chromatography. The start positions and migrated positions of ATP and free phosphoric acid are indicated as ‘Origin’, ‘³²P-ATP’, and ‘³²P-Pi’, respectively, on the right side of the figure. The data represent three independent experiments. Treatment with DMSO corresponds to ‘0′. (B) Gel mobility shift assay for RNA-binding activity of NS3 helicase. The reaction was carried out with 0.5 nM labeled ssRNA at the indicated concentrations of C-29EA or DMSO. The reaction mixture was subjected to gel mobility shift assay. (C) The relative RNA-binding ability was calculated with band densities in each lane and presented as a percentage of RNA-NS3 in the total density. The data represent three independent experiments. Treatment with DMSO corresponds to ‘0′.