NVP-BEZ235 inhibited cell proliferation in NPC cells.

<p>(A) Inhibitory effects of NVP-BEZ235 on NPC cells and normal nasopharyngeal cells. Cells were cultured at 8,000∼10,000 cells/well in 96-well plates and cell growth was assessed by MTT methods after 3 days of treatment with NVP-BEZ235 (0–5000 nM).The results were expressed as inhibitory rates of cells. n = 3,<b>*</b><i>P</i><0.05, <b># </b><i>P</i><0.01, inhibitory rates of the corresponding concentration from NPC cells <i>vs.</i> NP69 cells. The inhibitory rate was calculated by following formula: A = (1-X/Y) ×100%. A, inhibitory rate; X, the average absorbance values of experimental groups; Y, the average absorbance values of control groups. (B) Inhibitory effects of NVP-BEZ235 on NPC cells with treatment for 7 days. Cells were cultured at 2,000∼4,000 cells/well in 96-well plates and cell growth was assessed by MTT methods after 7 days of treatment with NVP-BEZ235 (0–2000 nM). <b>*</b><i>P</i><0.05, <b>#</b><i>P</i><0.01, inhibitory rates of the corresponding concentration from other NPC cells <i>vs.</i> CNE1 cells. (C) Inhibitory effects of NVP-BEZ235 on CNE2 and HONE1 cells with treatment for 14 days. Cells were cultured at 500∼1,000 cells/well in 96-well plates and cell growth was assessed by MTT methods after 14 days of treatment with NVP-BEZ235 (0–1000 nM).