Effect of siRNA suppression of C1GalT on expressions of the cellular TF, Tn, sialyl-Tn and Core 3 glycans in SW620 cells.

<p><b>A</b>: SW620 glycan expression in cell response to C1GalT siRNA or control siRNA. After treatment of the cells with C1GalT siRNA or control non-targeting siRNA (con-siRNA), cellular expressions of TF, Tn, sialyl-Tn and GSL-II binding (GlcNAc-, Core 3-associated glycans) were assessed by slot blots with monoclonal antibodies against TF, Tn, sialy-Tn or with biotin-GSL-II. Parallel blots were probed with antibody against β-actin for equal protein loading. Duplicate assessments are shown for each blot. <b>B</b>: Quantification of the expressions of cellular TF, Tn, sialy-Tn and GSL-II binding (GlcNAc-, Core 3-associated glycans) in SW620 cell response to siRNA C1GalT. Densities of the slots blots were quantified* and the glycan expressions are expressed as percentage change to the non-siRNA control after normalization with protein loading. *The blot densities of TF expression from untreated and C1GalT siRNA treated SW620 cells were 5144 and 834; Tn 1437 and 4313; sialyl-Tn 1748 and 4792; GSL-II binding (Core 3) 481 and 1229 and tublin 1984 and 1982.