Knockdown of glutamate cysteine ligase catalytic subunit by siRNA.

<p>(A) GCLC mRNA levels in A549 cells following 24 hours transfected with negative control siRNA, GCLC siRNA-1, GCLC siRNA-2 and GCLC siRNA-3. GCLC siRNA significantly decreased the GCLC mRNA levels in A549 cells. (B) Representative Western blot gel documents for GCLC and summarized data showing that efficiency of gene silencing of GCLC by siRNA. Cytosolic proteins were isolated from transfected cells. GCLC protein levels in cell extracts were measured by Western blot analysis and were normalised to β-actin expression levels. *** P<0.001, compared to negative control.