SDS-PAGE analysis of the purification of scFv-IC16 via Ni-NTA and Aβ1-16 GB1 NHS sepharose affinity chromatography.

Silke Dornieden (394650)
Andreas Müller-Schiffmann (394651)
Heinrich Sticht (173676)
Nan Jiang (21252)
Yeliz Cinar (394652)
Michael Wördehoff (394653)
Carsten Korth (217597)
Susanne Aileen Funke (314515)
Dieter Willbold (111480)

Publication date

March 2013

DOI

10.1371/journal.pone.0059820.g001

Abstract

<p>1 µl of the lysate and 15 µl of the purified fractions were applied to 16% polyacrylamide gels <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059820#pone.0059820-Laemmli1" target="_blank">[56]</a>. Lane 1: cell lysate, lane 2: eluate after affinity chromatography via Ni NTA–Agarose, lane 3: eluate after Aβ1-16-GB1-NHS-Sepharose affinity chromatography. Target protein is more than 99% pure after final purification step.</p