Purification of MBP-linker-MPR-TM.
- Zhen Gong (292434)
- Jose M. Martin-Garcia (122746)
- Sasha M. Daskalova (785648)
- Felicia M. Craciunescu (785649)
- Lusheng Song (785650)
- Katerina Dörner (785651)
- Debra T. Hansen (785652)
- Jay-How Yang (785653)
- Joshua LaBaer (30167)
- Brenda G. Hogue (785654)
- Tsafrir S. Mor (387810)
- Petra Fromme (326487)
Publication date
August 2015
DOIAbstract
<p>(A) Ni-affinity chromatogram of the βDDM extraction. Blue curve: UV absorbance at 280 nm; green curve: percentage of buffer B. (B) SDS-PAGE analysis of the flowthrough peaks and elution peak of the Ni-affinity chromatography. (C) SEC of the elution from Ni-affinity chromatography. Blue curve: UV absorbance at 280 nm. Peak A1 eluted at 11.1 mL and peak A2 eluted at 16.3 mL. (D) Silver stained SDS-PAGE (left) and anti-His Western blot (right) analysis of the peak A1 and A2 from SEC. L: molecular weight ladder.</p
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