Lectin affinity chromatography.

<p>A: Typical fractionation chromatogram of 1 gram IVIG on a 90 mL SNA-agarose column, showing the SNA non-binding (-SNA IVIG) flowthrough fraction (FT) and the SNA binding fractions (+SNA IVIG) eluate 1 (E1) and eluate 2 (E2). B: SDS PAGE and C: Lectin Blot (SNA) analysis of the SNA-purified IVIG fractions. IVIG: source IVIG before fractionation, FT: SNA non-binding flowthrough, E1: SNA binding eluate 1, E2: SNA binding eluate 2, NAase IVIG: Neuraminidase treated (desialylated) IVIG, M: molecular marker, red.: gel run under reducing conditions, non red.: gel run under non-reducing conditions.