Longitudinal analysis of SIVgag-specific CD8 T cells in seven Mamu A*01 rhesus macaques infected with SIVmac239.

<p>The gating strategy and representative flow cytometry showing the percentage (Mean +/− S.D.) of PD-1 expressing p11CM⁺ CD8⁺ T cell (Ki67<b>⁺</b> vs Ki67⁻) in PBMC at 14 and 112 dpi is shown in (A). The frequency of p11CM⁺ CD8⁺T cells and their PD-1 expression in PBMC (B), lymph node (C) and colorectal tissues (D) at 14, 42, 112 dpi are shown. The frequency of Ki67⁻ PD-1<b>⁺</b>, Ki67⁺ PD-1<b>⁺</b> and Ki67⁺ PD-1⁻ cells in p11CM⁺ CD8⁺ T cell in PBMC (E), lymph node (F), and colorectal tissues (G) following SIVmac239 infection are shown. The relative density of PD-1 expression (MFI) by p11CM⁺ CD8⁺T cells is plotted as a function of proliferative capacity based on Ki67 expression in PBMC (H), lymph node (I), and colorectal tissues (J) at 14, 42, and 112 dpi. PBMC, lymph node, and colorectal cell samples from Seven 7 Mamu-A*001 animals were included in these analyses.