High frequencies of MDSCs were found in bone marrow, liver, and PBMC, but very few in the LNs of the chronically SIV-infected macaques.

<p>(A) The gating strategy of CD14⁺MDSCs and Lin^- CD15⁺MDSCs from the SIV-infected bone marrow. The same gating strategy was used for other compartments. (B) The kinetics of plasma VLs in the 12 SIV_mac251-infected macaques showed persistent viral infection (mean±SEM). Single suspension cells from eight compartments of 12 SIVmac251 chronically (14 months-) infected Indian rhesus macaques were used to measure the frequencies of CD14⁺ MDSCs (C), Lin^- CD15⁺MDSCs (D), gag-specific CM9 Dextramer⁺ (E), and Ki67⁺ (F) in CD8⁺ T cells. In C-D, P<0.05 for bone marrow vs. all the other compartments except liver and PBMC; P<0.05 for liver vs. axillary LN, mesenteric LN, and colon LP; P<0.05 for PBMC vs. axillary LN, mesenteric LN, and colon LP in both types of MDSCs after Dunn’s multiple comparison tests. In E, P<0.05 for PBMC vs. axillary LN, mesenteric LN, spleen and bone marrow after Dunn’s multiple comparison tests.