Virological and immunological analyses in macaque R09-009 following CD8⁺ cell depletion.

<p>(A) Changes in peripheral CD8⁺ T-cell counts after the initial anti-CD8 antibody administration. Group N macaque, R09-009, was administered anti-CD8 antibody at week 108 post-infection and on days 3, 7, and 10 after the first administration. (B) Changes in plasma viral loads. (C) Changes in CD8⁺ T-cell responses specific for SIV Gag, Nef, Vif, Vpx, Vpr, Tat, Rev, Pol, and Env. (D) CD8⁺ T-cell responses specific for SIV Gag_206–216, Gag_241–249, Gag_367–381, Vif_114–124, Nef_9–19, Nef_89–97, and Nef_193–203 epitopes at week 113 post-infection. (E) Dominant non-synonymous mutations in plasma viral cDNA regions encoding Gag, Vif, and Nef epitopes. Viral <i>gag</i>, <i>vif</i>, and <i>nef</i> cDNA fragments were amplified from plasma RNA obtained at weeks 110 and 118 post-infection. Amino acid substitutions around SIV Gag_206–216, Gag_241–249, Gag_367–381, Vif_114–124, Nef_9–19, Nef_89–97, and Nef_193–203 epitopes are shown.