Cellular senescence is activated in <i>PTEN</i>-deficient cells.

<p>Percentage of β- galactosidase positive cells was determined following radiation (<b>A</b>) and rucaparib (<b>B</b>),treatment in <i>PTEN</i>-deficient LNCaP, C4-2, and PC3 cells. The percentage of β-galactosidase-positive cells was determined by counting ≥five different fields (∼70 cells/sample). <b>C</b>, Synergy analyses for senescence in LNCaP, PC3, and C4-2 cells for the combination treatment. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060408#pone.0060408.s002" target="_blank">Figs. S2</a> and 3 for β-galactosidase staining and additional synergy analyses. <b>D</b>, Percentage of β-galactosidase positive C4-2 cells was determined after 12 days of treatment with radiation ± rucaparib. <b>E</b>, parental and <i>TMPRSS2-ERG</i> fusion gene-expressing PC3 cells were quantified for SA-β-galactosidase staining after 6 days following 4 Gy radiation, 2.5 µM rucaparib, and the DNA-PKcs inhibitor NU7441 (500 nM), alone or in combination. Error bars represent SD of mean (n = 3).