PTEN deficiency is required for irradiation-induced EMT.

<p>(A) Radiation significantly decreases the expression of PTEN in the KESE-150/RR cells no matter in mRNA level (data presented as mean ±SD, **<i>P</i> <0.01) and protein level (representative western blots). (B) The transfected efficiency of siPTEN in KYSE-150 (left) and pcDNA-PTEN in KYSE-150/RR cells (right) (data presented as mean ±SD, **<i>P</i> <0.01). (C) Representative western blot analysis showed expression of PTEN, E-cadherin and vimentin in KYSE-150 cells transfected with siPTEN or vehicle, or KYSE-150/RR cells transfected with pcDNA-PTEN or pcDNA3.0. Data shown represent three different experiments. (D) Representative images of migration and invasion assay of KYSE-150 cells transfected with siPTEN or vehicle after 48 h (left); Summary graphs is for migration and invasion (right, data shown as mean ±SD, *<i>P</i> <0.05). (E) Representative images of migration assay and invasion assay of KYSE-150/RR cells transfected with pcDNA-PTEN or pcDNA3.0 were photographed after 24 h with crystal violet stain (left); Summary graphs for migration and invasion (right, data shown as mean ±SD, *<i>P</i> <0.05). siPTEN is short for siRNA-PTEN.