When cleavaging pcDNA3.1 (+)-CD, agarose gel electrophoresis analysis on cleavaged products revealed a band between 1.2 kb and 1.5 kb, which was consistent with target band (Fig. 2 A).

<p>When cleavaging pcDNA3.1(+)-CD -TNF-α, electrophoresis showed protein band at around 700 bp (Fig. 2 B). By cleavaging pcDNA 3.1(+)-TNF-α-IRES, respectively, TNF-α, IRES and CD were obtained (Fig. 2 C). M means DNA Marker and 1 mans pcDNA3.1(+)-CD/Xho Ι+Apa Ι in Fig. 2 A; M means DNA Marker and 1 mans pcDNA3.1(+)-CD -TNF-α/EcoR Ι+Not Ιin Fig. 2 B; 1 mans pcDNA 3.1(+)-TNF-α-IRES-CD/EcoRΙ+NotΙ, 2 means pcDNA 3.1(+)-TNF-α-IRES-CD/XhoΙ+NotΙ and 3 means pcDNA 3.1(+)-TNF-α-IRES-CD/XhoΙ+ApaΙ in Fig. 2 C.